Test Equipment Manuals Test Tubes Test/Measurement Test/Measurement - General Thermal Analysis Tissue. Berthold Lumat LB 9507 Tube Luminometer. Detect and identify Lumat LB 9507 Ultra Sensitive Tube. Weight 14 Kg Order information Order Number Lumat LB 9507, manual model. BERTHOLD TECHNOLOGIES. Synthesis and characterization of mannosylated pegylated polyethylenimine as a. (Acros, Fair Lawn. Product manual and using a Lumat LB 9507 (Berthold. LB 9507 Preface I Preface Use and Function The Lumat LB 9507 is a semi-automatic luminometer for universal use in bio- and chemiluminescence, and is very easy to operate.

The luminometer was tested using water samples and provided consistent sample readings with the included software and paired PC. At a Glance -Fully functional, giving consistent data (Data accuracy not validated.) -Paired with laptop and software -Great Cosmetic condition -Includes: Luminometer, ICE Software, Laptop (Fresh Windows XP Install), Technical Evaluation NLS acquired software and loaded in on a laptop with a fresh Windows install to test the Berthold Luminometer. The technician did not have consumables to use, but distilled and tap water served as samples. The trials produced repeatable results, but the technician was unable to confirm if the readouts were appropriate, lacking the reagents and precise knowledge to process the data points.

Condition The Berthold tube luminometer is in excellent cosmetic condition and demonstrated functionality.

Regulation of gene expression using small interfering RNA (siRNA) is a promising strategy for research and treatment of numerous diseases. In this study, we develop and characterize a delivery system for siRNA composed of polyethylenimine (PEI), polyethylene glycol (PEG), and mannose (Man). Cationic PEI complexes and compacts siRNA, PEG forms a hydrophilic layer outside of the polyplex for steric stabilization, and mannose serves as a cell binding ligand for macrophages. The PEI-PEG-mannose delivery system was constructed in two different ways.

In the first approach, mannose and PEG chains are directly conjugated to the PEI backbone. In the second approach, mannose is conjugated to one end of the PEG chain and the other end of the PEG chain is conjugated to the PEI backbone. The PEI-PEG-mannose delivery systems were synthesized with 3.45-13.3 PEG chains and 4.7-3. Winpcsign Basic. 0 mannose molecules per PEI.

The PEI-PEG-Man-siRNA polyplexes displayed a coarse surface in Scanning Electron Microscopy (SEM) images. Polyplex sizes were found to range from 169 to 357 nm. Gel retardation assays showed that the PEI-PEG-mannose polymers are able to efficiently complex with siRNA at low N/P ratios. Confocal microscope images showed that the PEI-PEG-Man-siRNA polyplexes could enter cells and localized in the lysosomes at 2h post-incubation. Pegylation of the PEI reduced toxicity without any adverse reduction in knockdown efficiency relative to PEI alone. Mannosylation of the PEI-PEG could be carried out without any significant reduction in knockdown efficiency relative to PEI alone.

Conjugating mannose to PEI via the PEG spacer generated superior toxicity and gene knockdown activity relative to conjugating mannose and PEG directly onto the PEI backbone. Regulation of gene expression using small interfering RNA (siRNA) is a promising strategy for research and treatment of numerous diseases. In this study, we develop and characterize a delivery system for siRNA composed of polyethylenimine (PEI), polyethylene glycol (PEG), and mannose (Man). Cationic PEI complexes and compacts siRNA, PEG forms a hydrophilic layer outside of the polyplex for steric stabilization, and mannose serves as a cell binding ligand for macrophages. The PEI-PEG-mannose delivery system was constructed in two different ways.

In the first approach, mannose and PEG chains are directly conjugated to the PEI backbone. In the second approach, mannose is conjugated to one end of the PEG chain and the other end of the PEG chain is conjugated to the PEI backbone. The PEI-PEG-mannose delivery systems were synthesized with 3.45 – 13.3 PEG chains and 4.7 – 3.0 mannose molecules per PEI.

The PEI-PEG-Man-siRNA polyplexes displayed a coarse surface in Scanning Electron Microscopy (SEM) images. Polyplex sizes were found to range from 169nm to 357nm.

Gel retardation assays showed that the PEI-PEG-mannose polymers are able to efficiently complex with siRNA at low N/P ratios. Confocal microscope images showed that the PEI-PEG-Man-siRNA polyplexes could enter cells and localized in the lysosomes at 2 hours post-incubation. Pegylation of the PEI reduced toxicity without any adverse reduction in knockdown efficiency relative to PEI alone. Mannosylation of the PEI-PEG could be carried out without any significant reduction in knockdown efficiency relative to PEI alone. Conjugating mannose to PEI via the PEG spacer generated superior toxicity and gene knockdown activity relative to conjugating mannose and PEG directly onto the PEI backbone.